Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine

Food and Agricultural Immunology

Wei Jiang¹  Lu Zeng¹  Hua Kuang¹  Shanshan Song¹  Liqiang Liu¹ 

[1] State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People’s Republic of Chin;International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People’s Republic of Chin;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu, People’s Republic of Chin;

关键词: Clorprenaline;    antibody;    pig urine;    immunochromatographic strip;   

DOI  :  10.1080/09540105.2017.1411469

来源: publisher

【 摘 要 】

A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104–1.818 ng/mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples.

【 授权许可】

CC BY