Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium

Food and Agricultural Immunology

Shuzhen Zou¹  Hua Kuang¹  Shanshan Song¹  Jianping Zhu¹  Liqiang Liu¹ 

[1] State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People’s Republic of Chin;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu, People’s Republic of Chin;

关键词: Cadmium;    immunochromatographic strip;    monoclonal antibody;    gold-nanoparticle (GNP);   

DOI  :  10.1080/09540105.2017.1354358

来源: publisher

【 摘 要 】

A novel and highly sensitive immunochromatographic strip based on a monoclonal antibody (3A9) was developed for the detection of cadmium in tap water. The 50% inhibition concentration of the antibody, which showed no cross-reactivity with other heavy metal ions, was 0.45 ng/mL and it recognized Cd(II)–1-(4-isothiocyanobenzyl) ethylenediamine-N,N,N′,N′-tetraacetic acid (EDTA) (Cd(II)–ITCBE) and not metal-free ITCBE and EDTA. The cutoff value for semi-quantitative detection of the strip was 5 ng/mL and the lower limit of detection for quantitative detection was 0.2 ng/mL using a scanning reader. The percent recovery ranged from 107.6% to 132% in tap water samples.

【 授权许可】

CC BY