A Highly Sensitive ELISA and Immunochromatographic Strip for the Detection of Salmonella typhimurium in Milk Samples

Sensors

Wenbin Wang²  Liqiang Liu²  Shanshan Song²  Lijuan Tang²  Hua Kuang²  Chuanlai Xu¹ 

[1] State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;

关键词: Salmonella typhimurium;    flagellin;    LPS;    monoclonal antibody;    sandwich ELISA;    immunochromatographic strip;    detection;   

DOI  :  10.3390/s150305281

来源: mdpi

【 摘 要 】

Murine monoclonal antibodies to target Salmonella typhimurium flagellin and lipopolysaccharide (LPS) were prepared and characterized. For the immunological detection of S. typhimurium, different pairs of monoclonal antibodies (MAbs) were tested in a sandwich enzyme linked immunosorbent assay (ELISA) format. After comparison, a sandwich ELISA and immunochromatographic strip based on LPS MAbs was established to detect S. typhimurium. The determination limits of the immunochromatographic strip in phosphate-buffered saline (PBS) containing 0.1% Tween 20 (PBST) and pure milk sample were found to be 1.25 × 10⁵ colony-forming units (cfu)/mL and 1.25 × 10⁶ cfu/mL S. typhimurium, respectively. Results can be obtained with the naked eye in 10 min. Cross-reactivity was observed with Salmonella paratyphi B, but not S. paratyphi A or Salmonella enteritidis. The LPS MAbs based immunochromatographic strip is rapid and convenient to detect S. typhimurium in milk samples.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.