Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides 1

FEBS Letters

Chaby, Richard²  Caroff, Martine²  Aussel, Laurent²  Kelly, John¹  Le Blay, Karine²  Perry, Malcolm B.¹  Thibault, Pierre¹ 

[1] Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada;Equipe ‘Endotoxines’, UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, F-91405 Orsay, France

关键词: Endotoxin;    Structure;    Mass spectrometry;    Serology;    Bordetella hinzii;    CE;    capillary electrophoresis;    ELISA;    enzyme-linked immunosorbent assay;    ES/MS;    electrospray mass spectrometry;    FAB;    fast atom bombardment;    Fuc2NAc4NMe;    2-acetamido-4-N-methyl-2;    4;    6-deoxy-galactose;    GalNA;    galactosaminuronic acid;    GlcA;    glucuronic acid;    GlcNAc;    N-acetyl glucosamine;    GC;    gas chromatography;    Hep;    heptose;    LPS;    lipopolysaccharide;    mAb;    monoclonal antibody;    MALDI;    matrix-assisted laser desorption/ionization;    Man2NAc3NAcA;    2-acetamido-3-acetamido-2;    3-dideoxy-mannuronic acid;    NMR;    nuclear magnetic resonance;    PDMS;    plasma desorption mass spectrometry;    PEA;    phosphoryl ethanolamine;    PPEA;    pyrophosphoryl ethanolamine;   

DOI  :  10.1016/S0014-5793(00)02181-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results: Mass spectrometry and serology revealed that the O-chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species.

【 授权许可】

Unknown