Novel variation of lipid A structures in strains of different Yersinia species

FEBS Letters

Perry, Malcolm B¹  Thérisod, Hélène³  Caroff, Martine³  Aussel, Laurent³  Bruneteau, Maud²  Karibian, Doris³ 

[1] Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada;Université Claude Bernard Lyon 1, UMR 5557 du Centre National de la Recherche Scientifique, F-69622 Villeurbanne, France;Equipe ‘Endotoxines’, UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, F-91405 Orsay, France

关键词: Endotoxin;    Lipid A;    Structure;    Plasma desorption mass spectrometry;    Yersinia;    GlcN;    glucosamine;    C14OH;    hydroxytetradecanoic acid;    C12;    dodecanoic acid;    C14;    tetradecanoic acid;    C16;    hexadecanoic acid;    C16:1;    hexadecenoic acid;    ara-4N;    aminoarabinose;   

DOI  :  10.1016/S0014-5793(99)01722-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Yersinia genus includes human and animal pathogens (plague, enterocolitis). The fine structures of the endotoxin lipids A of seven strains of Yersinia enterocolitica, Yersinia ruckeri and Yersinia pestis were determined and compared using mass spectrometry. These lipids differed in secondary acylation at C-2′: this was dodecanoic acid (C₁₂) for two strains of Y. enterocolitica and Y. ruckeri, tetradecanoic acid (C₁₄) in two other Y. enterocolitica and hexadecenoic acid (C_16:1) in Y. pestis. The enterocolitica lipids having a mass identical to that of Escherichia coli were found to be structurally different. The results supported the idea of a relation between membrane fluidity and environmental adaptability in Yersinia.

【 授权许可】

Unknown