Chemical structure of lipid A from porphyromonas (bacteroides) gingivalis lipopolysaccharide

FEBS Letters

Ogawa, Tomohiko¹ 

[1] Department of Oral Microbiology, Osaka University Faculty of Dentistry, Yamadaoka, Suita-Osaka 565, Japan

关键词: Endotoxin;    Lipid A;    Purification;    Structure;    Porphyromonas gingivalis;    Ac;    acetyl;    COSY;    correlation spectroscopy;    FAB;    fast atom bombardment;    GLC;    gas-liquid chromatography;    HPLC;    high performance liquid chromatography;    LPS;    lipopolysaccharides;    MS;    mass spectrometry;    NMR;    nuclear magnetic resonance spectroscopy;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(93)80512-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The novel chemical structure of Porphyromonas (Bacteroides) gingivalis strain 381 lipid A was determined to be a glucosamine β-(1–6) disaccharide 1-monophosphate acylated by 3-hydroxy-15-methylhexadecanoic acid and 3-hexadecanoyloxy-15-methylhexadecanoic acid at the 2- and 2'-positions, respectively. The absence of ester-linked phosphate at the 4'-position and fatty acids at the 3- and 3'-positions, and the presence of fatty acids possessing 16–17 carbon atoms are unique features, differentiating the lipid A from enterobacterial and other lipid As. These structural features may be related to its low endotoxic activity. Furthermore, P. gingivalis lipid A as well as its LPS stimulated the splenocytes from C3H/HeN and C3H/HeJ mice.

【 授权许可】

Unknown