Helicobacter mustelae lipid A structure differs from that of Helicobacter pylori

FEBS Letters

Perry, Malcolm B¹  Thérisod, Hélène²  Caroff, Martine²  Monteiro, Mario A¹ 

[1] Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada;Equipe ‘Endotoxines’, UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, F-91405 Orsay, France

关键词: Endotoxin;    Lipid A;    Structure;    Helicobacter mustelae;    BHI;    brain heart infusion;    GC;    gas chromatography;    GlcN;    glucosamine;    HMQC;    heteronuclear multiple quantum correlation;    LPS;    lipopolysaccharide;    MALDI;    matrix-assisted laser desorption ionization;    MS;    mass spectrometry;    NMR;    nuclear magnetic resonance;    PDMS;    plasma desorption mass spectrometry;    TLC;    thin layer chromatography;    TOF;    time of flight;   

DOI  :  10.1016/S0014-5793(01)02496-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lipid A structure of the Gram-negative bacterium Helicobacter mustelae, a ferret gastric pathogen responsible for the onset of gastric diseases in its host, was investigated. Two variant lipid A structures were found in the same strain. One structure contained a bisphosphorylated β-(1→6)-linked D-glucosamine backbone disaccharide with hydroxytetradecanoic acid in amide linkages. Unlike the structure described for the lipid A of the related human Helicobacter pylori gastric pathogen, which contains a C1 phosphate moiety, this lipid A presented phosphate groups at both the C1 and C4′ positions, and contained no octadecanoyl fatty acid, which is present in H. pylori. The second lipid A structure had a different fatty acid composition in that 3-OH C₁₆ replaced most of the amide-linked 3-OH C₁₄.

【 授权许可】

Unknown