Structure of the Bordetella pertussis 1414 endotoxin

FEBS Letters

Martin, Adèle¹  Caroff, Martine²  Brisson, Jean-Robert¹  Karibian, Doris² 

[1] Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada;Equipe ‘Endotoxines’, UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, F-91405 Orsay, France

关键词: Endotoxin;    Structure;    Matrix-assisted laser desorption/ionization mass spectrometry;    Nuclear magnetic resonance;    Bordetella pertussis;    COSY;    correlated spectroscopy;    TOCSY;    total correlated spectroscopy;    NOESY;    nuclear Overhauser enhancement spectroscopy;    HSQC;    heteronuclear single quantum coherence;    HMBC;    heteronuclear multiple bond connectivity;    LPS;    lipopolysaccharide;    MALDI;    matrix-assisted laser desorption/ionization mass spectrometry;    PPEA;    pyrophosphorylethanolamine;    Fuc2NAc4NMe;    2-acetamido-4-N-methyl-2;    4;    6-deoxy-galactose;    FucNAcMe;    2-acetamido-2-N-methyl-2;    6-deoxy-galactose;    Glc;    glucose;    GlcA;    glucuronic acid;    GlcNAc;    N-acetyl glucosamine;    Hep;    heptose;    Kdo;    3-deoxy-D-manno-octulopyranosonic acid;    Man2NAc3NAcA;    2-acetamido-3-acetamido-2;    3-dideoxy-mannuronic acid;    d-ManH;    dehydromannitol;   

DOI  :  10.1016/S0014-5793(00)01720-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The endotoxin (lipopolysaccharide) of Bordetella pertussis, the agent of whooping cough, consists of a lipid A linked to a highly branched dodecasaccharide containing several acid and amino sugars. The elucidation of the polysaccharide structure was accomplished by first analyzing the structures of fragments obtained by hydrolysis and nitrous deamination and then piecing the fragments together. The fine structure of the antigenic distal pentasaccharide, presented here, was determined by chemical analyses as well as by high-resolution nuclear magnetic resonance and mass spectrometry. The complete structure was reconstituted and confirmed by matrix-assisted laser desorption/ionization mass spectrometry. The following structure was derived from the combined experimental data:

【 授权许可】

Unknown