Structural characterization of the O‐chain polysaccharide isolated from Bordetella avium ATCC 5086: variation on a theme

FEBS Letters

Perry, Malcolm B¹  Thérisod, Hélène²  Caroff, Martine²  Larocque, Suzon¹  Brisson, Jean-Robert¹ 

[1] Institute for Biological Sciences, NRC, Ottawa, ON, Canada;Equipe ‘Endotoxines’, UMR 8619 du CNRS, I.B.B.M.C., Université de Paris-Sud, F-91405 Orsay, France

关键词: Endotoxin;    Lipopolysaccharide;    O-chain;    Nuclear magnetic resonance;    Matrix-assisted laser desorption-ionization mass spectrometry;    Bordetella avium;    COSY;    correlated spectroscopy;    HMBC;    heteronuclear multiple bond correlation;    HSQC;    heteronuclear single quantum coherence;    HSQCTOCSY;    HSQC with TOCSY step;    MALDI;    matrix-assisted laser desorption-ionization;    NOESY;    nuclear Overhauser enhancement spectroscopy;    PS;    polysaccharide;    TLC;    thin-layer chromatography;    TOCSY;    total correlated spectroscopy;   

DOI  :  10.1016/S0014-5793(02)03843-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution ¹H and ¹³C NMR and MALDI studies showed the O-chain to be a polymer composed of 1,4-linked 2-acetamidino-3-[3-hydroxybutanamido]-2,3-dideoxy-β-D-glucopyranosyluronic acid residues.

【 授权许可】

Unknown