Endotoxin induces rat lung ribonuclease activity

FEBS Letters

Wright, Angélique¹  Clerch, Linda B.¹  Massaro, Donald¹ 

[1] The Lung Biology Laboratory, The Departments of Pediatrics and Medicine, Georgetown University Medical Center, Washington, DC 20007, USA

关键词: Ribonuclease;    Endotoxin;    Lipopolysaccharide;    RNA-binding;    Rat lung;   

DOI  :  10.1016/0014-5793(93)80937-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lipopolysaccharide (endotoxin), a component of Gram-negative bacteria, causes marked alterations in eukaryotic gene expression and cellular physiology. We show that within one hour of injection of endotoxin into adult rats there is an induction of ribonuclease activity in the lung. The degradation of RNA was prevented by treatment of the lung extract from endotoxin-injected rats with ribonuclease inhibitor (RNasin). We suggest that induction by endotoxin of ribonuclease activity is a novel mechanism by which cells could alter gene expression to meet an environmental challenge and caution that the presence of ribonuclease can hinder molecular biological analyses of tissue extracts from endotoxin-treated rats.

【 授权许可】

Unknown