Immunochromatographic strip for rapid detection of phenylethanolamine A

Food and Agricultural Immunology

Wei Jiang¹  Lu Zeng¹  Hua Kuang¹  Shanshan Song¹  Liqiang Liu¹ 

[1] State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People’s Republic of Chin;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu, People’s Republic of Chin;

关键词: Phenylethanolamine A;    immunochromatographic strip;    antibody;    swine;   

DOI  :  10.1080/09540105.2017.1364709

来源: publisher

【 摘 要 】

A monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of detection was 0.033 ng/mL, and its detection range 0.033–0.679 ng/mL. The recovery rate for PEA in swine urine was 84.5–90.8%, with good stability and accuracy. “Dry” immunoassay test strips were successfully prepared based on antibody 2G10. When the strip was tested in swine urine, the cutoff value for PEA was 5 ng/mL, with highly sensitivity. The method developed here can be used to test swine urine on-site for the rapid detection of PEA.

【 授权许可】

CC BY