Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model

Drug Delivery

Yuting Huang¹  Hairong Liu¹  Zheng Pan²  Mengdan Cao²  Feng Zhang²  Dengming Zhou²  Ke Liu²  Xuanchu Duan³ 

[1] College of Materials Science and Engineering, Hunan University, Changsha, Hunan, China;Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China;Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China;Aier School of Ophthalmology, Central South University, Changsha, Hunan, China;Changsha Aier Eye Hospital, Changsha, Hunan, Chin;

关键词: Drug delivery system;    PHBV;    rosiglitazone;    glaucoma filtration surgery;   

DOI  :  10.1080/10717544.2019.1648590

来源: publisher

【 摘 要 】

The aim of this study is to investigate the effects and toxicities of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV)-loading rosiglitazone on preventing scar formation after glaucoma filtration surgery (GFS) in the rabbit model. Rosiglitazone/PHBV drug delivery system was prepared via electrospinning. Release behavior of RSG/PHBV membrane was evaluated by high-performance liquid chromatography. The different concentration membranes were implanted under the conjunctiva of the rabbit’s eyes (RSG/PHBV groups). Also, MMC-soaked sponges were placed under the conjunctiva of the eyes (positive group) for 3 min. Intraocular pressures and bleb features were then assessed for 4 weeks postoperative. Bleb sections were stained with HE, Masson’s trichrome and α smooth muscle action (αSMA) immunohistochemistry. The protein expression of collagen I, αSMA, and connective tissue growth factor in the bleb area were then quantified. The following results were observed: (1) the concentration of rosiglitazone would not affect the morphology of RSG/PHBV membrane. (2) RSG/PHBV membrane would effective and safety prevent the formation of fibrosis after GFS in the rabbit model. Implantation of RSG/PHBV membrane prevents scar formation after GFS. What’s more, it ameliorated toxicity to conjunctiva and cornea compared with the placement of MMC. The RSG/PHBV membrane would be a more effectivity and safer strategy than MMC.

【 授权许可】

CC BY