Lipid based drug delivery systems: Kinetics by SANS

3rd International Conference on Small Angle Neutron Scattering dedicated to the 80 anniversary of Yu.M. Ostanevich

Uhríková, D.^1 ; Teixeira, J.^2 ; Hubík, L.^1 ; Búcsi, A.^1 ; Kondela, T.^1 ; Murugova, T.^3 ; Ivankov, O.I.^3

Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, Bratislava

832 32, Slovakia^1

Laboratoire Léon Brillouin (CEA/CNRS), CEA Saclay, Gif-sur-Yvette Cedex

91191, France^2

Frank Laboratory of Neutron Physics, JINR, Joliot-Curie 6, Moscow region, Dubna

141980, Russia^3

关键词: Acidic conditions;    Bilayer thickness;    Drug delivery system;    N ,N-Dimethyldodecylamine;    pH sensitive;    Responsivity;    Small angle x-ray diffractions;    Structural reorganization;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/848/1/012007/pdf
DOI  :  10.1088/1742-6596/848/1/012007

来源: IOP

【 摘 要 】

N,N-dimethyldodecylamine-N-oxide (C12NO) is a surfactant that may exist either in a neutral or protonated form depending on the pH of aqueous solutions. Using small angle X-ray diffraction (SAXD) we demonstrate structural responsivity of C12NO/dioleoylphospha-tidylethanolamine (DOPE)/DNA complexes designed as pH sensitive gene delivery vectors. Small angle neutron scattering (SANS) was employed to follow kinetics of C12NO protonization and DNA binding into C12NO/DOPE/DNA complexes in solution of 150 mM NaCl at acidic condition. SANS data analyzed using paracrystal lamellar model show the formation of complexes with stacking up to ∼32 bilayers, spacing ∼ 62 Å, and lipid bilayer thickness ∼37 Å in 3 minutes after changing pH from 7 to 4. Subsequent structural reorganization of the complexes was observed along 90 minutes of SANS mesurements.