Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin

Molecules

Tim Scharnweber²  Catarina Santos²  Ralf-Peter Franke²  Maria Margarida Almeida¹ 

[1] Department of Ceramic and Glass Engineering, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal; E-mails:;Institute for Biological Interfaces; Forschungszentrum Karlsruhe GmbH, 76344 Eggenstein-Leopoldshafen Karlsruhe, Germany

关键词: Hydroxyapatite;    Spray dried particles;    5-Fluorouracil;    in Vitro cytotoxicity;    Drug delivery system;   

DOI  :  10.3390/molecules13112729

来源: mdpi

【 摘 要 】

In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17/2.8 cells indicating that ROS 17/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.