Lipid membranes loaded with Ca2+ and Zn2+ cations

3rd International Conference on Small Angle Neutron Scattering dedicated to the 80 anniversary of Yu.M. Ostanevich

Kuerka, N.^1,2 ; Dushanov, E.^2 ; Kholmurodov, K.T.^2,3 ; Katsaras, J.^4 ; Uhríková, D.^1

Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University, Bratislava

83232, Slovakia^1

Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna-Moscow Region

141980, Russia^2

Dubna International University for Society, Nature and Man, Dubna-Moscow region

141980, Russia^3

Joint Institute for Neutron Sciences, Oak Ridge National Laboratory, Oak Ridge

TN

37831-6453, United States^4

关键词: Bi-layer;    Bilayer thickness;    Biomimetic membrane;    Dipalmitoyl phosphatidylcholine;    Divalent metal ion;    Lipid membranes;    Multilamellar;    Small angle neutron;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/848/1/012008/pdf
DOI  :  10.1088/1742-6596/848/1/012008

来源: IOP

【 摘 要 】

We have studied the interactions of calcium and zinc with the biomimetic membrane made of dipalmitoyl-phosphatidylcholine (DPPC). The small angle neutron experiment on oriented multilamellar samples showed clearly differences in the effects of the two cations. For both, a bilayer thickness increases due to divalent metal ion (Me2+) binding, reaching the maximum at stoichiometry Me2+:DPPC∼1:7 mol/mol. However, while the further increase in Ca2+results in a bilayer thinning down to the level of pure DPPC, the Zn2+binding indicates the behaviour of a typical isotherm, reaching a level of saturation.