Mathematical Model of the Biosensors Acting in a Trigger Mode

Sensors

Romas Baronas²  Juozas Kulys¹ 

[1] Vilnius Gediminas Technical University, Sauletekio Avenue 11, 2040 Vilnius, Lithuania;Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, 2006 Vilnius, Lithuania

关键词: Biosensor;    amperometry;    modelling;    simulation;    amplification;   

DOI  :  10.3390/s40400020

来源: mdpi

【 摘 要 】

A mathematical model of biosensors acting in a trigger mode has been developed. One type of the biosensors utilized a trigger enzymatic reaction followed by the cyclic enzymatic and electrochemical conversion of the product (CCE scheme). Other biosensors used the enzymatic trigger reaction followed by the electrochemical and enzymatic product cyclic conversion (CEC scheme). The models were based on diffusion equations containing a non-linear term related to Michaelis-Menten kinetics of the enzymatic reactions. The digital simulation was carried out using the finite difference technique. The influence of the substrate concentration, the maximal enzymatic rate as well as the membrane thickness on the biosensor response was investigated. The numerical experiments demonstrated a significant gain (up to dozens of times) in biosensor sensitivity when the biosensor response was under diffusion control. In the case of significant signal amplification, the response time with triggering was up to several times longer than that of the biosensor without triggering.

【 授权许可】

Unknown   
© 2004 by MDPI (http://www.mdpi.org).