Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination

Sensors

Dainius Simelevicius¹  Romas Baronas¹ 

[1] Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania; E-Mail:

关键词: modelling;    simulation;    reaction-diffusion;    biosensor;    synergistic substrates;   

DOI  :  10.3390/s120404897

来源: mdpi

【 摘 要 】

In this paper the operation of an amperometric biosensor producing a chemically amplified signal is modelled numerically. The chemical amplification is achieved by using synergistic substrates. The model is based on non-stationary reaction-diffusion equations. The model involves three layers (compartments): a layer of enzyme solution entrapped on the electrode surface, a dialysis membrane covering the enzyme layer and an outer diffusion layer which is modelled by the Nernst approach. The equation system is solved numerically by using the finite difference technique. The biosensor response and sensitivity are investigated by altering the model parameters influencing the enzyme kinetics as well as the mass transport by diffusion. The biosensor action was analyzed with a special emphasis to the effect of the chemical amplification. The simulation results qualitatively explain and confirm the experimentally observed effect of the synergistic substrates conversion on the biosensor response.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.