Impedance Spectroscopy Technique for DNA Hybridization

Sensors

S. Hleli¹  A. Abdelghani¹ 

[1] ¹Unité de Recherche de Physique des Semiconducteurs, IPEST, La Marsa, 2070 Tunis, Tunisia ²Institut National des Sciences Appliquées et de Technologies, Centre Urbain Nord, Bp676, 1080 Charguia Cedex, Tunisia

关键词: Impedance Spectroscopy;    DNA;    Oligonucleotide;   

DOI  :  10.3390/s31000472

来源: mdpi

【 摘 要 】

The development of biosensors for detection and identification of DNA sequences by hybridization may reduce assay time and allow direct quantitation of the target. This article describes the use of impedance spectroscopy technique for digoxigeninthiol- labeled ssDNA probe immobilization, anti-digoxigenin binding and targeted DNA hybridization. The analysis of the impedance spectra in terms of equivalents circuits of the gold/electrolyte interface and gold/digoxigenin-thiol-labeled/electrolyte interface is discussed. The DNA hybridization shows a variation in the impedance spectra.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.