A 19F NMR study of the interaction of 3‐fluoro‐4‐demethoxydaunomycin with the hexanucleotide d(TCCGGA)2

FEBS Letters

Warrener, Ronald N.³  Russell, Richard A.²  Collins, J.Grant¹  Barr, Kathryn E.¹ 

[1] Department of Chemistry, University College, University of New South Wales, Australian Defence Force Academy, ACT 2600, Australia;School of Chemistry, Deakin University, Geelong, Vic. 3217, Australia;School of Chemistry, University College of Central Queensland, Rockhampton, Qld. 4702, Australia

关键词: 19F NMR;    Anthracycline;    Sequence specificity;    Oligonucleotide;    DNA;   

DOI  :  10.1016/0014-5793(93)81562-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹⁹F NMR spectroscopy has been used to study the binding of the fluorinated anthracycline 3-fluoro-4-demethoxydaunomycin with the hexanucleotide d(TCCGGA)₂. In the spectrum of the 1:1 anthracycline-d(TCCGGA)₂ complex four resonances of approximately equal intensity were observed. This indicated that 4-demethoxydaunomycin intercalated at all possible sites with similar affinity. This suggests that the specific high affinity binding sites that are observed in anthracycline—DNA footprinting experiments are strongly regulated by the local DNA conformation.

【 授权许可】

Unknown