Modulation of DNA methyltransferase during the life cycle of a mealybug Planococcus lilacinus

FEBS Letters

Brahmachari, Vani¹  Devajyothi, C.¹ 

[1] Department of Microbiology and Cell Biology, ICMR Centre for Genetics and Cell Biology, Indian Institute of Science, Bangalore 560 012, India

关键词: Chromosome imprinting;    Fertilization;    DNA methyltransferase;    Sequence specificity;    Synthetic polymer;   

DOI  :  10.1016/0014-5793(89)80705-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The levels of de novo DNA methyltransferase were studied during the development of a mealybug, Planococcus lilacinus. No enzyme activity could be detected in extracts from second instar females. But the enzyme occurs at high levels in third instar females and is maintained at that level during fourth instar when gametogenesis, fertilization and chromosome imprinting occur. These results suggest a developmental stage-specific modulation of levels of DNA methyltransferase. Assays with synthetic polymers showed that the enzyme can methylate not only polymers containing GpG but also those containing CpA and CpI.

【 授权许可】

Unknown