The DNA sequence specificity of cyanomorpholinoadriamycin

FEBS Letters

Phillips, Don R.¹  Cullinane, Carleen¹ 

[1] Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia

关键词: Cyanomorpholinoadriamycin;    DNA;    Intrastrand crosslink;    Transcription;    Sequence specificity;   

DOI  :  10.1016/0014-5793(91)81185-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cyanomorpholinoadriamycin (1 μM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior to 5′-CC (six) and 5′-GG (six) sequences of the non-template strand, consistent with the presence of intrastrand crosslinking between adjacent guanine residues. Minor levels of blockage were at 5′-GC and 5′-CG sequences and may reflect low levels of interstrand crosslinking.

【 授权许可】

Unknown