期刊论文详细信息
FEBS Letters | |
Specific transcription of Orthopox virus DNA by HeLa cell RNA polymerase II | |
Malcolm, A.D.B.2  Johnson, K.2  Archard, L.C.1  | |
[1] Virology Division, Department of Medical Microbiology St. Mary's Hospital Medical School, London W2, England;Department of Biochemistry, St. Mary's Hospital Medical School, London W2, England | |
关键词: Orthopoxvirus; DNA; Transcription; RNA polymerase II; | |
DOI : 10.1016/0014-5793(85)80041-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A HeLa cell extract was used to transcribe DNA isolated from cowpox virus. Truncated templates generate accurately initiated run-off transcripts of discrete sizes and whose sensitivity to inhibition by α-amanitin indicates synthesis by cell RNA polymerase II. A mapped restriction fragment of wild-type cowpox DNA contains specific sites of initiation which are not detected in the geographically equivalent fragment from a cowpox mutant having a defined sequence rearrangement in this region.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020287335ZK.pdf | 303KB | download |