Specific transcription of Orthopox virus DNA by HeLa cell RNA polymerase II

FEBS Letters

Malcolm, A.D.B.²  Johnson, K.²  Archard, L.C.¹ 

[1] Virology Division, Department of Medical Microbiology St. Mary's Hospital Medical School, London W2, England;Department of Biochemistry, St. Mary's Hospital Medical School, London W2, England

关键词: Orthopoxvirus;    DNA;    Transcription;    RNA polymerase II;   

DOI  :  10.1016/0014-5793(85)80041-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A HeLa cell extract was used to transcribe DNA isolated from cowpox virus. Truncated templates generate accurately initiated run-off transcripts of discrete sizes and whose sensitivity to inhibition by α-amanitin indicates synthesis by cell RNA polymerase II. A mapped restriction fragment of wild-type cowpox DNA contains specific sites of initiation which are not detected in the geographically equivalent fragment from a cowpox mutant having a defined sequence rearrangement in this region.

【 授权许可】

Unknown