Effects of solute conditions on the relative affinities of the oligonucleotides d(G‐C)5 and d(A‐T)5 for the anthracycline drug 2‐fluoro‐4‐demethoxydaunomycin

FEBS Letters

Warrener, Ronald N.¹  Russell, Richard A.²  Collins, J.Grant²  Hammer, Brendon C.² 

[1] Department of Chemistry, The Faculties, Australian National University, PO Box 4, Canberra, ACT 2600, Australia;Department of Chemistry, University College, University of New South Wales, Australian Defence Force Academy, Canberra, ACT 2600, Australia

关键词: NMR;    19F-;    Daunomycin;    Adriamycin;    Sequence specificity;    DNA;    Oligonucleotide;   

DOI  :  10.1016/0014-5793(89)81198-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹⁹F NMR has been used to show that changes in NaCl concentration, as well as the presence of lysine or arginine, affect the equilibrium distribution of the synthetic anthracycline 2-fluoro-4-demethoxydaunomycin (2FD) between binding sites on d(G-C)₅ and d(A-T)₅ in a 1:1:1 molar aqueous system: 2FD/d(G-C)₅/d(A-T)₅. Varying the pH between 6.2 and 7.7 had no effect. NaCl concentrations below 0.1 M led to a d(G-C)₅ preference while above 0.1 M a preference for d(A-T)₅ was observed. At comparable solute concentrations, use of either lysine or arginine resulted in a significant drug preference for d(G-C)₅ compared to systems containing only NaCl.

【 授权许可】

Unknown