Absorption mode two‐dimensional NOE spectroscopy of exchangeable protons in oligonucleotides

FEBS Letters

Bax, Ad¹  Brooks, Bernard R.³  Sklenár̆, Vladimír¹  Zon, Gerald² 

[1] >Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA;Applied Biosystems, 850 Lincoln Center Dr., Forster City, CA 94404, USA;Division of Computer Research and Technology, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Oligonucleotide;    DNA;    2D NMR;    Nuclear Overhauser effect;    Water suppression;    Hydrogen exchange;   

DOI  :  10.1016/0014-5793(87)80699-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new NMR method is described for the generation of absorption mode two-dimensional NOE spectra of oligonucleotides in H₂O solution. The method yields spectra that are free of baseline distortions with excellent suppression of the intense H₂O resonance. The method is demonstrated for a sample of the dodecamer d(CGCGAATTCGCG)₂. All exchangeable base protons are identified and a number of new types of NOE connectivities are observed.

【 授权许可】

Unknown