Direct observation of (H8,H6)‐Hr J‐coupling correlations in oligonucleotides for unambiguous resonance assignments

FEBS Letters

Hosur, R.V.¹  Gundhi, Praful¹  Chary, K.V.R.¹ 

[1] Tata Institute of Fundamental Research, Bombay 400 005, India

关键词: J-scaled COSY;    2D NMR;    Oligonucleotide;   

DOI  :  10.1016/0014-5793(85)81000-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four-bond H8/H6-H1 scalar coupling correlations in two-dimensional correlated spectroscopy have been observed directly for the first time by using the J-scaled COSY [(1985) Chem. Phys. Lett. 116, 105-108] technique in a dinucleotide, cytidylyl(2'–5')guanosine (CpG). Unambiguous resonance assignment of nonexchangeable protons in CpG has been obtained using these H8/H6-H1' 4-bond correlations and the various 3- and 4-bond sugar ring proton correlations observed in the COSY and SUPER COSY experiments.

【 授权许可】

Unknown