Determination of Trace Level of cAMP in Locusta Migratoria Manilensis Meyen by HPLC with Fluorescence Derivation

International Journal of Molecular Sciences

Lujun Zhang¹  Huiting Zhang¹  Jinfang Wang¹ 

[1] College of Sciences, China Agricultural University, Beijing 100094, China

关键词: cAMP;    OA;    Locusta migratoria manilensis Meyen;    HPLC;    HPLC-MS;    fluorescence derivation;    DMHA;    TBAS.;   

DOI  :  10.3390/i7080266

来源: mdpi

【 摘 要 】

A sensitive and rapid method was developed for the determination of cAMP inLocusta migratoria manilensis Meyen by high-performance liquid chromatography withfluorescence detection. The cAMP was derivatized using chloroacetaldehyde and TBASbuffer/methanol was used as the mobile phase. A detection quantification of 40 fmol/mlcould be achieved when using fluorescence detection. An HPLC-MS method using DMHAas an ion-pair agent to analyze cAMP was also demonstrated. We studied the effect ofdopamine and other stimulants on cAMP levels from isolated locust central nervoussystems. The new method is well suited for the analysis of cAMP in small biologicalsamples.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.