Zeatin is indispensable for the G2‐M transition in tobacco BY‐2 cells

FEBS Letters

Witters, Erwin³  Inzé, Dirk¹  Dewitte, Walter³  Laureys, Françoise³  Van Onckelen, Harry³  Van Montagu, Marc² 

[1] Laboratoire Associé de l'Institut National de la Recherche Agronomique (France) and Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Rijksuniversiteit Gent, B-9000 Ghent, Belgium;Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, B-9000 Ghent, Belgium;Laboratorium voor Plantenbiochemie en -fysiologie, Department of Biology, Universiteit Antwerpen (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium

关键词: G2-M transition;    Cytokinin;    Synchronisation;    Lovastatin;    Tobacco BY-2 cell;    Aϕ;    aphidicolin;    cAMP;    3′;    5′-cyclic adenosine monophosphate;    BA;    6-benzyl aminopurine;    2;    4-D;    2;    4-dichlorophenoxyacetic acid;    DAPI;    4;    6′-diamidino-2-phenylindole;    DZ;    dihydrozeatin;    DZNG;    dihydrozeatin-N-glucoside;    DZMP;    dihydrozeatin riboside-5′-monophosphate;    DZOG;    dihydrozeatin-O-glucoside;    DZROG;    dihydrozeatin riboside-O-glucoside;    DZR;    dihydrozeatin riboside;    ES;    electrospray;    gfw;    gram fresh weight;    GiP;    isopentenyladenine glucoside;    HMG-CoA;    3-hydroxy-3-methylglutaryl coenzyme A;    HPLC;    high performance liquid chromatography;    2iP;    isopentenyladenine;    2iPA;    isopentenyladenosine;    iPAP;    isopentenyladenosine-phosphate;    2iP9G;    isopentenyladenine-9-glucoside;    MA;    mevalonic acid;    MI;    mitotic index;    MS;    mass spectrometry;    NAA;    α-naphtyl acetic acid;    NH4Ac;    ammoniumacetate;    PBS;    phosphate-buffered saline;    TBY-2;    tobacco Bright Yellow 2;    Z;    zeatin;    Z9G;    zeatin-9-glucoside;    ZMP;    zeatin riboside-5′-monophosphate;    ZNG;    zeatin-N-glucoside;    ZOG;    zeatin-O-glucoside;    ZR;    zeatin riboside;    ZROG;    zeatin riboside-O-glucoside;   

DOI  :  10.1016/S0014-5793(98)00297-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

LC-MS/MS quantification of endogenous cytokinins proved that lovastatin affects cytokinin biosynthesis by inhibiting HMG-CoA reductase. Out of eight different aminopurines and a synthetic auxin tested for their ability to override lovastatin inhibition of mitosis, only zeatin was active. Our data point to a key role for a well-defined cytokinin (here, zeatin) in the G₂-M transition of tobacco BY-2 cells.

【 授权许可】

Unknown