Retention of folate receptors on the cytoskeleton of Dictyostelium during development

FEBS Letters

Newell, P.C.¹  Tillinghast, H.S.¹ 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England

关键词: Folate receptor;    Cytoskeleton;    Deaminofolate;    Distribution-free plot;    Dictyostelium;    cAMP;    adenosine 3';    5'-cyclic monophosphate;    FA;    folic acid;    DAFA;    2-deamino-2-hydroxy folic acid;    HPLC;    high-pressure liquid chromatography;   

DOI  :  10.1016/0014-5793(84)81189-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A revised folate binding assay was employed to demonstrate the presence of folate receptors on vegetative Dictyostelium discoideum amoebae. These receptors have a dissociation constant of 300 nM and are present at 45000 per cell. We found a pool of receptors attached to the cytoskeleton of vegetative amoebae and these receptors (10000 sites/cell, K _d480 nM) remain associated with the cytoskeleton through the first 12 h of development. We discuss the possible roles for these retained receptors in later D. discoideum development.

【 授权许可】

Unknown