Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro

International Journal of Molecular Sciences

Liefeng Zhang¹  Hui Jiang¹  Wenjie Zhu¹  Lin Wu¹  Lingling Song¹  Qiuyan Wu¹ 

[1] Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210097, P.R. Chin

关键词: Protection;    insulin;    degradation;    casein;    protamine;    cyclodextrin;   

DOI  :  10.3390/ijms9122376

来源: mdpi

【 摘 要 】

Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-casein or HP-β-CD-protamine, most insulin was protected from degradation by intestinal enzymes. In addition, it was found that the complexation of insulin with HP-β-CD was characterized by UV absorption spectra. These results indicated that HP-β-CD, casein and protamine could offer some positive and useful results, and could protect insulin from degradation during their transit through the intestinal tract.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).