Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability

International Journal of Molecular Sciences

Liefeng Zhang¹  Wenjie Zhu¹  Lingling Song²  Yifan Wang²  Hui Jiang²  Suyun Xian² 

[1]Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, Nanjing Normal University, Nanjing 210097, P.R. China

关键词: Protection;    insulin;    stability;    cyclodextrin;   

DOI  :  10.3390/ijms10052031

来源: mdpi

【 摘 要 】

The objective of this study was to elucidate the effects of hydroxylpropyl-β-cyclodextrin (HP-β-CD) on the in vitro stability of insulin. It was found that HP-β-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-β-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through ¹H-NMR experiments it was found that the protective effect of HP-β-CD was due to complexation with insulin. The results suggest that the presence of HP-β-CD could improve the stability of insulin in different environments.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.