Emodin-8-O-β-D-Glucoside from Polygonum Amplexicaule D. Don var. Sinense Forb. Promotes Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

Molecules

Mei-Xian Xiang¹  Zong Xu¹  Han-Wen Su¹  Jinyue Hu¹ 

[1] ¹College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China ²College of Pharmacy, South-Central University for Nationalities, Wuhan 430075, China ³Renmin Hospital, Wuhan University, Wuhan 430060, China

关键词: emodin-8-O-β-D-glucoside;    Polygonum amplexicaule D. Don var. sinense Forb. (Polygonaceae);    proliferation;    differentiation;    osteoblastic MC3T3-E1 cell;   

DOI  :  10.3390/molecules16010728

来源: mdpi

【 摘 要 】

Polygonum amplexicaule D. Don var. sinense Forb. (Polygonaceae) (PAF) is a famous traditional herb used to treat fractures, rheumatoid arthritis, muscle injury and pain. The present study was designed to investigate a PAF derived-chemical compound emodin-8-O-β-D-glucoside (EG) on the proliferation and differentiation of osteoblastic MC3T3-E1 cell in vitro. A compound was isolated from PAF extract by HPLC and identified as emodin-8-O-β-D-glucoside (EG) by spectroscopic methods. EG significantly promoted cell proliferation at 0.1–100 ng/mL, and increased the cell proportion in S-phase from 16.34% to 32.16%. Moreover, EG increased alkaline phosphatase (ALP) expression in MC3T3-E1 cells at the concentration from 0.1 to 100 ng/mL and inhibited PGE₂ production induced by TNF-α in osteoblasts at the concentrations ranging from 10–100 ng/mL, suggesting that cell differentiation was induced in MC3T3-E1 osteoblasts. Taken together, these results indicated compound EG directly stimulated cell proliferation and differentiation of osteoblasts, therefore this study preliminarily explored the pharmacological mechanism of PAF to promote the healing of bone rheumatism and various fractures.

【 授权许可】

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