MicroRNA-199a-5p Affects Porcine Preadipocyte Proliferation and Differentiation

International Journal of Molecular Sciences

Xin-E Shi¹  Yue-Feng Li¹  Long Jia¹  Hong-Lei Ji¹  Zi-Yi Song¹  Jia Cheng²  Guo-Fang Wu¹  Cheng-Chuang Song¹  Qiang-Ling Zhang¹  Jia-Yu Zhu¹ 

[1] Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; E-Mails:;Vitamin D Research Institute, Shaanxi University of Technology, Hanzhong 723000, China; E-Mail:

关键词: porcine preadipocyte;    miR-199a-5p;    proliferation;    differentiation;   

DOI  :  10.3390/ijms15058526

来源: mdpi

【 摘 要 】

MicroRNAs (miRNAs), a class of small non-coding RNAs, have emerged as novel and potent regulators of adipogenesis. However, few miRNAs have been fully investigated in porcine adipogenesis, given the fact that pig is not only an apropos model of human obesity research, but also a staple meat source of human diet. In this study, we showed that miRNA-199a-5p is highly expressed in porcine subcutaneous fat deposits compared to several other tissue types and organs measured alongside. Overexpression of miR-199a-5p in porcine preadipocytes significantly promoted cell proliferation while attenuating the lipid deposition in porcine adipocytes. By target gene prediction and experimental validation, we demonstrated that caveolin-1 (Cav-1) may be a bona fide target of miR-199a-5p in porcine adipocytes, accounting for some of miR-199a-5p’s functions. Taken together, our data established a role of miR-199a-5p in porcine preadipocyte proliferation and differentiation, which is at least partially played by downregulating Cav-1.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland