Chemical Profile and Biological Potential of Non-Polar Fractions from Centroceras clavulatum (C. Agardh) Montagne (Ceramiales, Rhodophyta)

Molecules

Otávio P. Rocha¹  Rafael De Felício¹  Ana Helena B. Rodrigues¹  Daniela L. Ambrósio¹  Regina Maria B. Cicarelli¹  Sérgio De Albuquerque¹  Maria Claudia M. Young¹  Nair S. Yokoya¹ 

[1] ¹Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto – USP, Av. do Café, s/n, CEP 14040-903, Monte Alegre, Ribeirão Preto-SP, Brazil

关键词: Centroceras clavulatum;    GC-MS analysis;    Rhodophyta;    trypanocidal activity;    antifungal activity;   

DOI  :  10.3390/molecules16087105

来源: mdpi

【 摘 要 】

The present study reports the Gas Chromatography-Mass Spectrometry (GC-MS) evaluation of the hexanes and dichloromethane fractions from extracts of the red alga Centroceras clavulatum (C. Agardh) Montagne. Twenty three compounds were identified, totaling ca. 42% of both fractions (0.18 g mass extract). The main constituents of the fractions were hexadecanoic acid (17.6%) and pentadecanoic acid (15.9%). Several secondary metabolites with interesting biological activity, such as (-)-loliolide, neophytadiene, phytol were identified. In addition, several classes of secondary metabolites, including phenolic compounds (e.g., phenylacetic acid), terpene derivatives, fatty acids, halogenated compound (e.g., 2-chlorocyclohexenol), lignoids, steroids, esters, amides (e.g., hexadecanamide), ketones, carboxylic acids, aldehydes and alcohols were observed. The occurrence of several of these structural classes is described for the first time in this species. The same fractions analyzed by GC-MS, and a separate set of polar fractions, were evaluated against two life cycle stages (epimastigote and trypomastigote forms) of the protozoan Trypanosoma cruzi and against phytopatogenic fungi Cladosporium cladosporiodes and C. sphaerospermum. The dichloromethane fraction was active against both T. cruzi forms (epimastigote IC₅₀ = 19.1 μg.mL⁻¹ and trypomastigote IC₅₀ = 76.2 μg.mL⁻¹). The hexanes and ethyl acetate fractions also displayed activity against both fungi species (200 μg) by TLC-bioautography.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.