Protective Effects of Sodium Selenite against Aflatoxin B1-Induced Oxidative Stress and Apoptosis in Broiler Spleen

International Journal of Environmental Research and Public Health

Fengyuan Wang¹  Gang Shu¹  Xi Peng¹  Jing Fang¹  Kejie Chen²  Hengmin Cui²  Zhengli Chen²  Zhicai Zuo²  Junliang Deng²  Yi Geng² 

[1] Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China;

关键词: aflatoxin b₁;    sodium selenite;    oxidative stress;    apoptosis;    spleen;   

DOI  :  10.3390/ijerph10072834

来源: mdpi

【 摘 要 】

The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B₁-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB₁ (AFB₁ group), 0.3 mg/kg AFB₁ + 0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB₁ + 0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB₁ + 0.6 mg/kg Se (+Se group III), respectively. According to biochemical assays, AFB₁ significantly decreased the activities of glutathione peroxidase, total superoxide dismutase, glutathione reductase, catalase and the level of glutathione hormone, while it increased the level of malondialdehyde. Moreover, AFB₁ increased the percentage of apoptosis cells by flow cytometry and the occurrence of apoptotic cells by TUNEL assay. Simultaneous supplementation with sodium selenite restored these parameters to be close to those in control group. In conclusion, sodium selenite exhibited protective effects on AFB₁-induced splenic toxicity in broilers by inhibiting oxidative stress and excessive apoptosis.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.