Effects of Dietary Selenium on Histopathological Changes and T Cells of Spleen in Broilers Exposed to Aflatoxin B1

International Journal of Environmental Research and Public Health

Kejie Chen¹  Xi Peng¹  Jing Fang¹  Hengmin Cui¹  Zhicai Zuo²  Junliang Deng²  Zhengli Chen²  Yi Geng²  Weimin Lai²  Li Tang² 

[1] Department of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China;

关键词: sodium selenite;    aflatoxin b₁;    histological lesion;    T-cell subsets;    flow cytometry;    broilers;   

DOI  :  10.3390/ijerph110201904

来源: mdpi

【 摘 要 】

Aflatoxin B₁ (AFB₁), which causes hepatocellular carcinoma and immune-suppression, is commonly found in feedstuffs. To evaluate the ability of selenium (Se) to counteract the deleterious effects of AFB₁, two hundred 1-day-old male avian broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB₁ (AFB₁ group), 0.3 mg/kg AFB₁+0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB₁+0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB₁+0.6 mg/kg Se (+Se group III), respectively. Compared with control group, the relative weight of spleen in the AFB₁ group was decreased at 21 days of age. The relative weight of spleen in the three +Se groups was higher than that in the AFB₁ group. By pathological observation, the major spleen lesions included congestion in red pulp and vacuoles appeared in the lymphatic nodules and periarterial lymphatic sheath in the AFB₁ group. In +Se groups II and III, the incidence of major splenic lesions was decreased. The percentages of CD₃⁺, CD₃⁺CD₄⁺ and CD₃⁺CD₈⁺ T cells in the AFB₁ group were lower than those in control group from 7 to 21 days of age, while there was a marked increase in the three +Se groups compared to the AFB₁ group. The results indicated that sodium selenite could improve the cellular immune function impaired by AFB₁ through increasing the relative weight of spleen and percentages of splenic T cell subsets, and alleviating histopathological spleen damage.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.