Achyranthes bidentata Polypeptides Reduces Oxidative Stress and Exerts Protective Effects against Myocardial Ischemic/Reperfusion Injury in Rats

International Journal of Molecular Sciences

Ru Tie³  Lele Ji³  Ying Nan²  Wenqing Wang¹  Xiangyan Liang³  Fei Tian³  Wenjuan Xing⁴  Miaozhang Zhu⁴  Rong Li⁵ 

[1] Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China; E-Mail:;Department of Physiology, Xi’an Medical University, Xi’an 710021, China; E-Mail:;Experiment Teaching Center, Fourth Military Medical University, Xi’an 710032, China; E-Mails:;Department of Physiology, Fourth Military Medical University, Xi’an 710032, China; E-Mail:;Department of Geratology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

关键词: Achyranthes bidentata polypeptides;    oxidative stress;    myocardial ischemia/reperfusion;    apoptosis;   

DOI  :  10.3390/ijms141019792

来源: mdpi

【 摘 要 】

Achyranthes bidentata, a Chinese medicinal herb, is reported to be neuroprotective. However, its role in cardioprotection remains largely unknown. Our present study aimed to investigate the effects of Achyranthes bidentata polypeptides (ABPP) preconditioning on myocardial ischemia/reperfusion (MI/R) injury and to test the possible mechanisms. Rats were treated with ABPP (10 mg/kg/d, i.p.) or saline once daily for one week. Afterward, all the animals were subjected to 30 min of myocardial ischemia followed by 4 h of reperfusion. ABPP preconditioning for one week significantly improved cardiac function following MI/R. Meanwhile, ABPP reduced infarct size, plasma creatine kinase (CK)/lactate dehydrogenase (LDH) activities and myocardial apoptosis at the end of reperfusion in rat hearts. Moreover, ABPP preconditioning significantly inhibited superoxide generation, gp91^phox expression, malonaldialdehyde formation and enhanced superoxide dismutase activity in I/R hearts. Furthermore, ABPP treatment inhibited PTEN expression and increased Akt phosphorylation in I/R rat heart. PI3K inhibitor wortmannin blocked Akt activation, and abolished ABPP-stimulated anti-oxidant effect and cardioprotection. Our study demonstrated for the first time that ABPP reduces oxidative stress and exerts cardioprotection against MI/R injury in rats. Inhibition of PTEN and activation of Akt may contribute to the anti-oxidant capacity and cardioprotection of ABPP.

【 授权许可】

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© 2013 by the authors; licensee MDPI, Basel, Switzerland