Antioxidant Constituents of Cotoneaster melanocarpus Lodd

Antioxidants

Veronika M. D. Holzer²  Agnieszka D. Lower-Nedza²  Myagmar Nandintsetseg¹  Javzan Batkhuu¹ 

[1] Laboratory of Bioorganic Chemistry and Pharmacognosy, Department of Biochemistry and Bioorganic Chemistry, School of Biology and Biotechnology, National University of Mongolia, P.O.B-617, Ulaanbaatar-46A, Mongolia; E-Mails:;Department of Pharmacognosy, Institute of Pharmaceutical Sciences, Karl-Franzens University, Universitaetsplatz 4/I, Graz 8010, Austria; E-Mails:

关键词: Cotoneaster melanocarpus;    DPPH;    antioxidants;    TLC;    HPLC;    LC-MS;   

DOI  :  10.3390/antiox2040265

来源: mdpi

【 摘 要 】

The aim of this study was the evaluation of the antioxidant capacity of Cotoneaster melanocarpus Lodd. and the identification of antioxidant active constituents of this plant. C. melanocarpus Lodd. is a shrub indigenous to Mongolia and used in Traditional Mongolian Medicine as a styptic. Before extraction, the plant material was separated into three parts: young sterile shoots, older stems and leaves. All these parts were extracted with water, methanol, ethyl acetate, dichloromethane and hexane, successively. The methanolic extract of the sterile shoots showed the highest antioxidant activity in the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay (IC₅₀ 30.91 ± 2.97 µg/mL). This active extract was further analyzed with chromatographic methods. TLC fingerprinting and HPLC indicated the presence of the flavonol glycosides quercetin-3-O-rutinoside (rutin), quercetin-3-O-galactoside (hyperoside) and quercetin-3-O-glucoside (isoquercetin), ursolic acid as well as chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid. The findings were substantiated with LC-MS. All identified compounds have antioxidant properties and therefore contribute to the radical scavenging activity of the whole plant.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.