Free Radical Scavenging Activity and Characterization of Sesquiterpenoids in Four Species of Curcuma Using a TLC Bioautography Assay and GC-MS Analysis

Molecules

Jing Zhao¹  Jiang-sheng Zhang¹  Bin Yang¹  Guang-Ping Lv¹ 

[1] ¹Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China ²Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing, China ^†The authors contributed equally to this work.

关键词: Curcuma;    TLC;    DPPH;    GC-MS;    Free radical scavenging activity;   

DOI  :  10.3390/molecules15117547

来源: mdpi

【 摘 要 】

The sesquiterpenoids are one of major groups of antioxidants in Curcuma besides curcuminoids. However, the real substances contributing to the antioxidant activity are still unknown. In this paper, the antioxidant activity of sesquiterpenoids in four species and two essential oils from Curcuma genus was determined and compared based on TLC separation and DPPH bioautography assay. Their antioxidant capacities were quantitatively evaluated using densitometry with detection at 530 nm (λ_reference = 800 nm) using vitamin C as reference. The results showed that Curcuma longa rhizomes had the highest antioxidant capacity while C. phaeocaulis presented the lowest one among the four species of Curcuma. Moreover, essential oil of C. wenyujin showed higher antioxidant potential than that of C. longa. The main TLC bands with antioxidant activity of the four species of Curcuma were collected and characterized using GC-MS, and thus curzerene, furanodiene, α-turmerone, β-turmerone and β-sesquiphellandrene were determined as major sesquiterpenoids with antioxidant activity in Curcuma.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.