Antibacterial and Antibiofilm Activities of Tryptoquivalines and Meroditerpenes Isolated from the Marine-Derived Fungi Neosartorya paulistensis, N. laciniosa, N. tsunodae, and the Soil Fungi N. fischeri and N. siamensis

Marine Drugs

Nelson M. Gomes³  Lucinda J. Bessa³  Suradet Buttachon³  Paulo M. Costa³  Jamrearn Buaruang⁴  Tida Dethoup¹  Artur M.S. Silva² 

[1] Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10240, Thailand; E-Mail:;Departamento de Química, Universidade de Aveiro, Aveiro 4810-1933, Portugal; E-Mail:;ICBAS—Instituto de Ciências Biomédicas de Abel Salazar, Rua de Jorge Viterbo Ferreira, 228, Porto 4050-313, Portugal; E-Mails:;Division of Environmental Science, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand; E-Mail:

关键词: antibacterial;    antibiofilm;    multidrug-resistant;    tryptoquivalines;    meroditerpenes;    Neosartorya;    marine-derived fungi;   

DOI  :  10.3390/md12020822

来源: mdpi

【 摘 要 】

A new meroditerpene, sartorypyrone C (5), was isolated, together with the known tryptoquivalines l (1a), H (1b), F (1c), 3′-(4-oxoquinazolin-3-yl) spiro[1H-indole-3,5′]-2,2′-dione (2) and 4(3H)-quinazolinone (3), from the culture of the marine sponge-associated fungus Neosartorya paulistensis (KUFC 7897), while reexamination of the fractions remaining from a previous study of the culture of the diseased coral-derived fungus N. laciniosa (KUFC 7896) led to isolation of a new tryptoquivaline derivative tryptoquivaline T (1d). Compounds 1a–d, 2, 3, and 5, together with aszonapyrones A (4a) and B (4b), chevalones B (6) and C (7a), sartorypyrones B (7b) and A (8), were tested for their antibacterial activity against four reference strains (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa), as well as the environmental multidrug-resistant isolates. Only aszonapyrone A (4a) and sartorypyrone A (8) exhibited significant antibacterial activity as well as synergism with antibiotics against the Gram-positive multidrug-resistant strains. Antibiofilm assays of aszonapyrone A (4a) and sartorypyrone A (8) showed that practically no biofilm was formed in the presence of their 2× MIC and MIC. However, the presence of a sub-inhibitory concentration of ½ MIC of 4a and 8 was found to increase the biofilm production in both reference strain and the multidrug-resistant isolates of S. aureus.

【 授权许可】

CC BY   
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