Recombinant spider silk coatings functionalized with enzymes targeting bacteria and biofilms

MicrobiologyOpen

Fredrik Seijsing¹  Johan Seijsing¹  My Hedhammar²  Camilla Ståhl²  Oskar Öhman²  Linnea Nilebäck²  Rajeev Pasupuleti² 

[1] Department of Molecular Biosciences The Wenner‐Gren Institute Stockholm University Stockholm Sweden;Department of Protein Science School of Engineering Sciences in Chemistry, Biotechnology and Health KTH Royal Institute of Technology AlbaNova University Center Stockholm Sweden;

关键词: antibacterial;    antibiofilm;    coating;    endolysin;    recombinant spider silk;   

DOI  :  10.1002/mbo3.993

来源: DOAJ

【 摘 要 】

Abstract Bacteria forming biofilms on surgical implants is a problem that might be alleviated by the use of antibacterial coatings. In this article, recombinant spider silk was functionalized with the peptidoglycan degrading endolysin SAL‐1 from the staphylococcal bacteriophage SAP‐1 and the biofilm‐matrix‐degrading enzyme Dispersin B from Aggregatibacter actinomycetemcomitans using direct genetic fusion and/or covalent protein–protein fusion catalyzed by Sortase A. Spider silk assembly and enzyme immobilization was monitored using quartz crystal microbalance analysis. Enzyme activity was investigated both with a biochemical assay using cleavage of fluorescent substrate analogues and bacterial assays for biofilm degradation and turbidity reduction. Spider silk coatings functionalized with SAL‐1 and Disperin B were found to exhibit bacteriolytic effect and inhibit biofilm formation, respectively. The strategy to immobilize antibacterial enzymes to spider silk presented herein show potential to be used as surface coatings of surgical implants and other medical equipment to avoid bacterial colonization.

【 授权许可】

Unknown