Ionic Liquid-Based Vacuum Microwave-Assisted Extraction Followed by Macroporous Resin Enrichment for the Separation of the Three Glycosides Salicin, Hyperin and Rutin from Populus Bark

Molecules

Fengli Chen²  Kailin Mo¹  Zhaizhi Liu²  Fengjian Yang²  Kexin Hou²  Shuangyang Li²  Yuangang Zu² 

[1] Sichuan Academy of Forestry, Chengdu 610081, China; E-Mail:;Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; E-Mails:

关键词: ionic liquid vacuum microwave-assisted extraction (ILVMAE);    Populus bark;    macroporous resin;    glycoside;   

DOI  :  10.3390/molecules19079689

来源: mdpi

【 摘 要 】

An effective ionic liquid vacuum microwave-assisted method was developed for extraction of the thermo- and oxygen-sensitive glycosides salicin, hyperin and rutin from Populus bark due to the strong solvating effects of ionic liquids on plant cell walls. In this study, [C₄mim]BF₄ solution was selected as the extracting solution for extraction of the target analytes. After optimization by single factor experiments and response surface methodology, the optimum condition parameters were achieved, which included 1.0 M [C₄mim]BF₄, 2 h soaking time, −0.08 MPa vacuum, 20 min microwave irradiation time, 400 W microwave irradiation power and 25 mL/g liquid/solid ratio. Under the optimum conditions, higher extraction yields of salicin (35.53 mg/g), hyperin (1.32 mg/g) and rutin (2.40 mg/g) were obtained. Compared with other extraction methods, the developed method provided higher yields of the three target components after a relatively shorter extraction time (20 min). No obvious degradation of the target analytes was observed under the optimum conditions in performed stability studies and the proposed method had a high reproducibility. Meanwhile, after adsorption and desorption on macroporous D101 resin, the target analytes can be effectively separated from the [C₄mim]BF₄ ionic liquid extraction solution and the yields of salicin, hyperin and rutin were 89%, 82% and 84%, respectively. The recovered [C₄mim]BF₄ ionic liquid presented a good extraction effect on the three analytes after recycling five times.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.