Industrial-Scale Preparation of Akebia Saponin D by a Two-Step Macroporous Resin Column Separation

Molecules

Yue Wu¹  De Ji¹  Yunfei Liu¹  Chunfeng Zhang¹ 

[1] State Key Laboratory of Natural Products and Functions, China Pharmaceutical University, Ministry of Education, No. 24 Tongjiaxiang, Nanjing 210009, China

关键词: akebia saponin D;    Dipsaci Radix;    large-scale preparation;    macroporous resin;   

DOI  :  10.3390/molecules17077798

来源: mdpi

【 摘 要 】

A simple and efficient procedure for the industrial preparation of akebia saponin D, one of the bioactive compounds commonly found in the well-known Chinese Medicinal herb Dipsaci Radix, was developed. First, HPD-722 was selected from among 10 kinds of macroporous absorption resins. Following this step, the purity of akebia saponin D was increased about 10 times from 6.27% to 59.41%. In order to achieve a higher purity, ADS-7 was chosen from among five kinds of macroporous absorption resins, and the purity of akebia saponin D was increased from 59.41% to 95.05%. The result indicated HPD-722 and ADS-7 were the most suitable resins to purify akebia saponin D from Dipsaci Radix. Under these conditions, large-scale preparation of akebia saponin D was carried out successfully. The preparation method is simple, efficient, and has been demonstrated to be effective for large scale preparations of akebia saponin D from Dipsaci Radix.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.