Bioactivity-Guided Fractionation of Physical Fatigue-Attenuating Components from Rubus parvifolius L.

Molecules

Jianhong Chen¹  Xianfeng Wang¹  Yongqing Cai¹  Ming Tang¹  Qing Dai¹  Xiaogang Hu¹  Mingchun Huang¹  Fengjun Sun¹  Yao Liu¹ 

[1] Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing 400038, China;

关键词: Rubus parvifolius L.;    anti-fatigue;    macroporous resin;    weight-loaded swimming;   

DOI  :  10.3390/molecules180911624

来源: mdpi

【 摘 要 】

Alleviation of fatigue has been emerging as a serious issue that requires urgent attention. Health professionals and sports physiologists have been looking for active natural products and synthetic compounds to overcome fatigue in humans. This study was designed to define the anti-fatigue property of Rubus parvifolius L. (RPL) by characterization of active constituents using a mouse forced swimming test model. Four RPL fractions with different polarities containing anti-fatigue activity were sequentially isolated from the n-butanol RPL extract, followed by elution of 50% ethanol-water fraction from D101 macroporous resin chromatography to obtain nigaichigoside F₁, suavissimoside R₁ and coreanoside F₁. Active constituents of the 50% ethanol-water eluate of RPL were total saponins. The fractions were examined based on the effect on weight-loaded swimming capacity of mice. Serum levels of urea nitrogen (SUN), triglyceride fatty acids (TG), lactate dehydrogenase (LDH), lactic acid (LA), ammonia and hepatic glycogen (HG) were also examined for potential mechanisms underlying the anti-fatigue effect of RPL extracts. During the experiment, two inflammatory markers, interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) in serum, were measured. We found that total saponins from RPL possess potent capabilities to alleviate mouse fatigue induced by forced swimming and that nigaichigoside F₁ was responsible for the pharmacological effect. The underlying mechanisms include delays of SUN and LA accumulation, a decrease in TG level by increasing fat consumption, increases in HG and LDH so that lactic acid accumulation and ammonia in the muscle were reduced, and suppression of increased immune activation and inflammatory cytokine production. Our findings will be helpful for functional identification of novel anti-fatigue components from natural medicinal herbs.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.