Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901

International Journal of Molecular Sciences

Xiaoyan Zhu¹  Fenglei Luo¹  Yixiong Zheng³  Jiukai Zhang¹  Jianzhen Huang²  Chongde Sun¹  Xian Li¹ 

[1] Laboratory of Fruit Quality Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails:;Forestry Bureau of Ouhai, Wenzhou 325000, China; E-Mail:;Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; E-Mail:

关键词: macroporous resin;    high speed counter-current chromatograph (HSCCC);    Citrus reticulate;    poncirin;    purification;    gastric cancer;   

DOI  :  10.3390/ijms14058684

来源: mdpi

【 摘 要 】

Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland