Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway

Molecules

Kai Gao¹  Meiyou Liu¹  Jinyi Cao¹  Minna Yao¹  Yunyang Lu²  Jiankang Li¹  Xiaohe Zhu¹  Zhifu Yang¹  Aidong Wen¹ 

[1] Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China; E-Mails:;Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China; E-Mail:

关键词: Parkinson’s disease;    LBP;    PC cells;    apoptosis;    reactive oxygen species;    nitric oxide;    mitochondrion;   

DOI  :  10.3390/molecules20010293

来源: mdpi

【 摘 要 】

Oxidative stress plays an important role in Parkinson’s disease and other neurodegenerative disorders. Lycium barbarum polysaccharides (LBP), the main active ingredients extracted from the fruits of Lycium barbarum L., have been shown to be a potent antioxidant. In the present study, we investigated the protective effects, and the possible mechanism of action of LBP against 6-hydroxydopamine (6-OHDA)-induced apoptosis in PC12 cells. Our data demonstrated that LBP significantly reversed the 6-OHDA-induced decrease in cell viability, prevented 6-OHDA-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. Furthermore, LBP also slowed the accumulation of reactive oxygen species (ROS) and nitric oxide (NO), decreased the level of protein-bound 3-nitrotyrosine (3-NT) and intracellular free Ca²⁺, and inhibiting the overexpression of nuclear factor κB (NF-κB), neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS). These results demonstrate that LBP prevents 6-OHDA-induced apoptosis in PC12 cells, at least in part through the ROS-NO pathway.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.