International Journal of Molecular Sciences | |
MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1 | |
Weigong Zhao2  Caijun Wu3  Yanying Dong3  Yunfeng Ma3  Yaofeng Jin1  Yanhong Ji3  | |
[1] Department of Pathology, the Second Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710004, China; E-Mail:;Department of Orthopedics, the First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710061, China;Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China; E-Mails: | |
关键词: miR-24; differentiation; osteoblast; T-cell factor-1; | |
DOI : 10.3390/ijms160511699 | |
来源: mdpi | |
【 摘 要 】
MicroRNAs (miRNAs) have been reported to have diverse biological roles in regulating many biological processes, including osteogenic differentiation. In the present study, we identified that miR-24 was a critical regulator during osteogenic differentiation. We found that overexpression of miR-24 significantly inhibited osteogenic differentiation, which decreased alkaline phosphatase activity, matrix mineralization and the expression of osteogenic differentiation markers. In contrast, inhibition of miR-24 exhibited an opposite effect. Furthermore, we delineated that miR-24 regulates post-transcriptionals of T-cell factor-1 (Tcf-1) via targeting the 3'-untranslated region (UTR) of Tcf-1 mRNA. MiR-24 was further found to regulate the protein expression of Tcf-1 in the murine osteoprogenitors cells and bone mesenchymal stem cells. Additionally, the positive effect of miR-24 suppression on osteoblast differentiation was apparently abrogated by Tcf-1 silencing. Taken together, our data suggest that miR-24 participates in osteogenic differentiation by targeting and regulating Tcf-1 expression in osteoblastic cells.
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
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