A Specific Oligodeoxynucleotide Promotes the Differentiation of Osteoblasts via ERK and p38 MAPK Pathways

International Journal of Molecular Sciences

Xu Hou⁴  Yuqin Shen¹  Chao Zhang⁴  Liru Zhang⁴  Yanyan Qin¹  Yongli Yu²  Liying Wang³ 

[1] Department of Periodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China; E-Mails:;Department of Immunology, Medical College of Norman Bethune, Jilin University, Changchun 130021, China; E-Mail:;Department of Molecular Biology, Medical College of Norman Bethune, Jilin University, Changchun 130021, China; E-Mail:;Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China; E-Mails:

关键词: oligodeoxynucleotide;    osteoblast;    differentiation;    ERK1/2 MAPK;    p38 MAPK;   

DOI  :  10.3390/ijms13077902

来源: mdpi

【 摘 要 】

A specific oligodeoxynucleotide (ODN), ODN MT01, was found to have positive effects on the proliferation and activation of the osteoblast-like cell line MG 63. In this study, the detailed signaling pathways in which ODN MT01 promoted the differentiation of osteoblasts were systematically examined. ODN MT01 enhanced the expression of osteogenic marker genes, such as osteocalcin and type I collagen. Furthermore, ODN MT01 activated Runx2 phosphorylation via ERK1/2 mitogen-activated protein kinase (MAPK) and p38 MAPK. Consistently, ODN MT01 induced up-regulation of osteocalcin, alkaline phosphatase (ALP) and type I collagen, which was inhibited by pre-treatment with the ERK1/2 inhibitor U0126 and the p38 inhibitor SB203580. These results suggest that the ERK1/2 and p38 MAPK pathways, as well as Runx2 activation, are involved in ODN MT01-induced up-regulation of osteocalcin, type I collagen and the activity of ALP in MG 63 cells.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.