An Oligodeoxynucleotide with Promising Modulation Activity for the Proliferation and Activation of Osteoblast

International Journal of Molecular Sciences

Zhiyuan Feng⁵  Yuqin Shen³  Liying Wang⁴  Lin Cheng¹  Jing Wang⁵  Quanshun Li²  Wei Shi² 

[1] Department of Oral Cavity, The Second Affiliated Hospital of Shanxi Medical University, 382 Wuyi Road, Taiyuan 030001, China; E-Mail:;Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, 2699 Qianjin Road, Changchun 130021, China; E-Mail:;Department of Periodontal, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China; E-Mail:;Department of Molecular Biology, College of Basic Medicine, Jilin University, Changchun 130021, China; E-Mail:;Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China; E-Mails:

关键词: oligodeoxynucleotide;    osteoblast;    proliferation;    activation;    bone remodeling;   

DOI  :  10.3390/ijms12042543

来源: mdpi

【 摘 要 】

The paper explored the regulatory role of oligodeoxynucleotides (ODNs) with specific sequences in the proliferation and activation of osteoblast, using human osteoblast-like cell line MG 63 as the model. Through the administration of ODNs to MG 63 cells at a concentration of 1.0 μg/mL, ODN MT01 with positive effects on proliferation and activation of osteoblast was selected from 11 different ODNs by methyl thiazolyl tetrazolium (MTT) assay and alkaline phosphatase (ALP) activity measurement. To get a deeper insight into the molecular mechanism, effects of ODN MT01 treatment on the expression level of Sp7, runx-2, collagen-I, osteoprotegerin (OPG) and RANK ligand (RANKL) were determined using quantitative real time PCR and Western blotting. Remarkably, the mRNA and protein expression levels of Sp7, runx-2, collagen-I and OPG were improved after ODN MT01 treatment. Meanwhile, the protein expression level of RANKL was dramatically decreased. These results suggested that ODN MT01 had a significant impact in facilitating osteogenic proliferation and activation, and provided a direct evidence for the notion that single strand ODN could regulate the balance of bone formation and resorption, and thus was of great potential in the rebuilding of alveolar bone.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.