[125I]FIAU imaging in a preclinical model of lung infection: quantification of bacterial load

American Journal of Nuclear Medicine and Molecular Imaging

Mrudula Pullambhatla¹  Graham Beck¹  Bruno Jedynak¹  Jens U Wurthner¹  Jean Tessier¹  Martin G Pomper¹ 

关键词: Inflammation;    thymidine kinase;    nucleoside;    SPECT;    PET;    molecular imaging;   

DOI  :  

学科分类：过敏症与临床免疫学

来源: e-Century Publishing Corporation

【 摘 要 】

2'-Fluoro-2'-deoxy-1β-D-arabinofuranosyl-5-[¹²⁵I]iodouracil ([¹²⁵I]FIAU), a substrate for the thymidine kinase (TK) present in most bacteria, has been used as an imaging agent for single photon emission computed tomography (SPECT) in an experimental model of lung infection. Using SPECT-CT we show that [¹²⁵I]FIAU is specific for bacterial infection rather than sterile inflammation. We report [¹²⁵I]FIAU lung uptake values of 1.26 ± 0.20 percent injected dose per gram (%ID/g) in normal controls, 1.69 ± 0.32 %ID/g in lung inflammation and up to 7.14 ± 1.09 %ID/g in lung infection in ex vivo biodistribution studies at 24 h after intranasal administration of bacteria. Images of [¹²⁵I]FIAU signal within lung can be used to estimate the number of bacteria present, with a limit of detection of 10⁹ colony forming units per mL on the X-SPECT scanner. [¹²⁵I]FIAU-Based bacterial imaging may be useful in preclinical models to facilitate the development of new antibiotics, particularly in cases where a corresponding human trial is planned.

【 授权许可】

Unknown