Oral, intestinal, and skin bacteria in ventral hernia mesh implants

Journal of Oral Microbiology

Odd Langbach⁴  Morten Enersen⁵  Ola Røkke¹  Ingar Olsen²  Anne Karin Kristoffersen³  Emnet Abesha-Belay³ 

[1] Department of Gastroenterologic Surgery, Akershus University Hospital, Faculty of Medicine, University of Oslo, Norway;Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway;Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway;Akershus University Hospital;Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norwayb

关键词: ventral hernia;    mesh;    implants;    oral bacteria;    16S rRNA;    DNA;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Background:Inventralherniasurgery,meshimplantsareusedtoreducerecurrence.Infectionaftermeshimplantationcanbeaproblemandratesaround6–10%havebeenreported.Bacterialcolonizationofmeshimplantsinpatientswithoutclinicalsignsofinfectionhasnotbeenthoroughlyinvestigated.Moleculartechniqueshaveproveneffectiveindemonstratingbacterialdiversityinvariousenvironmentsandareabletoidentifybacteriaonagene-specificlevel.Objective:Thepurposeofthisstudywastodetectbacterialbiofilminmeshimplants,analyzeitsbacterialdiversity,andlookforpossibleresemblancewithbacterialbiofilmfromtheperiodontalpocket.Methods:Thirtypatientsreferredtoourhospitalforrecurrenceafterformerventralherniameshrepair,wereexaminedforperiodontitisinadvanceofnewsurgicalherniarepair.Oralexaminationincludedperiapicalradiographs,periodontalprobing,andsubgingivalplaquecollection.Apieceofmesh(1×1cm)fromtheabdominalwallwasharvestedduringthenewsurgicalherniarepairandanalyzedforbacteriabyPCRand16SrRNAgenesequencing.FrompatientswithpositivePCRmeshsamples,subgingivalplaquesampleswereanalyzedwiththesametechniques.Results:Agreatvarietyoftaxaweredetectedin20(66.7%)meshsamples,includingtypicaloralcommensalsandperiodontopathogens,enterics,andskinbacteria.Meshandperiodontalbacteriawerefurtheranalyzedforsimilarityin16SrRNAgenesequences.In17sequences,thelevelofresemblancebetweenmeshandsubgingivalbacterialcolonizationwas98–100%suggesting,butnotproving,atransferoforalbacteriatothemesh.Conclusion:Theresultsshowgreatbacterialdiversityonmeshimplantsfromtheanteriorabdominalwallincludingoralcommensalsandperiodontopathogens.Meshcanbereachedbybacteriainseveralwaysincludinghematogenousspreadfromanoralsite.However,othersitessuchasgutandskinmayalsoserveassourcesforthemeshbiofilm.Keywords:ventralhernia;mesh;implants;oralbacteria;16SrRNA;DNA(Published:29July2016)Citation:JournalofOralMicrobiology2016,8:31854-http://dx.doi.org/10.3402/jom.v8.31854

【 授权许可】

Unknown